Proteomics

Dataset Information

0

Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting


ABSTRACT: Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mirounga Angustirostris (ncbitaxon:9716)

SUBMITTER: Jane Khudyakov  

PROVIDER: MSV000088122 | MassIVE |

SECONDARY ACCESSION(S): PXD028629

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-06-12 | MSV000082455 | MassIVE
2012-10-21 | E-GEOD-40951 | biostudies-arrayexpress
2016-11-24 | E-MTAB-5027 | biostudies-arrayexpress
2013-10-09 | PXD000488 | Pride
2013-12-05 | PXD000596 | Pride
2024-02-20 | PXD047849 | Pride
2024-01-19 | MSV000093893 | MassIVE
2024-05-23 | PXD033079 | Pride
2019-04-05 | GSE103952 | GEO
2024-06-07 | PXD045439 | Pride