Nontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease

ABSTRACT: Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples were filtered. ~35 micrograms of each sample was separated by direction injection and microflow LC (1 mm x 100 mm Waters ACQUITY Premier; 100 uL/min) using a gradient of 3-28% acetonrile, and MS/MS was acquired on a Thermo Exploris 480 using a 120K MS1 scan and 30K MS2 with a staggered/overlapping method. Data analysis used Spectronaut 17. Study pool QC data, and individual files used for statistical analysis, are described in the accompanying metadata.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Allison E. Ashley-Koch  

PROVIDER: MSV000093893 | MassIVE | Fri Jan 19 09:54:00 GMT 2024

SECONDARY ACCESSION(S): PXD048716

REPOSITORIES: MassIVE