Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis of Bacteroides ovatus under varying GI conditions

ABSTRACT: Bacteroides ovatus were grown under varying conditions to test their viability under different GI conditions.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacteroides Ovatus (ncbitaxon:28116)

SUBMITTER: Anthony Haag

PROVIDER: MSV000088716 | MassIVE | Fri Jan 21 10:53:00 GMT 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression analyses of GI eosinophils under homeostatic conditions

Project description:Gene expression analyses of GI eosinophils under homeostatic conditions

| PRJNA416256 | ENA

Comparative Proteomic Analysis of Fucosylated Glycoproteins in Bacteroides thetaiotaomicron under Different Polysaccharide Nutrition Conditions

Project description:Bacteroides thetaiotaomicron, one of the most eminent representative gut commensal Bacteroides species, is able to use the L-fucose in host-derived and dietary polysaccharides to modify its capsular polysaccharides and glycoproteins through a mammalian-like salvage metabolic pathway. This process is essential for the colonization of the bacteria and for symbiosis with the host. However, despite the importance of fucosylated proteins (FGPs) in Bacteroide thetaiotaomicron, their types, distribution, and functions remain unclear. In this project, the effects of different saccharide (glucose, corn starch, mucin, and fucoidan) nutrition conditions on FGP expressions and fucosylation are investigated using a chemical biological method based on metabolic labeling and bioorthogonal reaction. According to the results of label-free quantification, 559 FGPs (205 downregulated and 354 upregulated) are affected by the dietary conditions. Of these differentially expressed proteins, 65 proteins show extremely sensitive fucosylation levels. Specifically, the fucosylation of the chondroitin sulfate ABC enzyme, Sus proteins, and cationic efflux system proteins varies significantly upon the addition of mucin, corn starch, or fucoidan. Moreover, these polysaccharides can trigger an appreciable increase in the fucosylation level of the two-component system and ammonium transport proteins. These results highlight the efficiency of the combined metabolic glycan labeling and bio-orthogonal reaction in enriching the intestinal Bacteroide glycoproteins. Moreover, it emphasize the sensitivity of Bacteroides fucosylation to polysaccharide nutrition conditions, which allows for the regulation of bacterial growth.

2022-02-08 | PXD023756 | Pride

Digital gene expression of WT and gi mutant under control and drought conditions in Arabidopsis

Project description:We report the application of digital gene expression analysis for high-throughput profiling the different gene expression of WT and gi mutant under control and drought conditions. Examination gene expression of WT and gi under control and drought conditions

2013-07-12 | E-GEOD-48810 | biostudies-arrayexpress

Bacteroides fragilis 638R

Project description:Growth of Bacteroides fragilis under nanaerobic conditions

| PRJNA630195 | ENA

Role of BmoR in Bacteroides fragilis response to oxidative stress

Project description:Comparison of transcriptional profile of wild-type and bmoR mutant strains of Bacteroides fragilis grown under anaerobic conditions and after exposure to atmospheric oxygen for 1 hour.

2018-02-01 | GSE104575 | GEO

Digital gene expression of WT and gi mutant under control and drought conditions in Arabidopsis

2013-07-12 | GSE48810 | GEO

Temperature shift under anaerobic conditions

Project description:Temperature shift under anaerobic conditions

| PRJNA507430 | ENA

On the role of DOM under varying climate change conditions in Crocosphaera

Project description:On the role of DOM under varying climate change conditions in Crocosphaera

| PRJNA952626 | ENA

Genome-wide RNA-seq of Bacteroides thetaiotaomicron VPI-5482 with Bacteroides phage challenge

Project description:Purpose: Examining the transcriptome of Bacteroides thetaiotaomicron VPI-5482 challenged with Bacteroides phage to assess surface molecule expression changes Methods: Bacteroides thetaiotaomicron was grown in BPRM in vitro or Germ-Free mice were monocolonized with Bacteroides thetaiotaomicron and gavaged with ARB25 phage. Fold change was calculated as live phage versus heat-killed phage treated samples with n=3 biological replicates. Once cells reached an optical density corresponding to mid-log phase growth (absorbance between 0.4-0.5), RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) using DEseq2 normalization with default parameters. Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >5-fold and with at least 2/3 biological replicates with a normalized expression level >1% of the overall average, and a p-value < 0.05 (t test with Benjamini-Hochberg correction) Results: Specific capsule expression was increased in wild-type B. thetaiotaomicron during phage infection in vitro and in vivo. Many corresponding in vivo genes were upregulated as well as other surface layer proteins.

2020-05-01 | GSE147071 | GEO

Sugarcane drought experiment under field conditions

Project description:Sugarcane drought experiment under field conditions

| PRJNA515077 | ENA