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Proteome reallocation enables the selective de novo biosynthesis of non-linear, branched-chain acetate esters

ABSTRACT: Proteome reallocation enables the selective de novo biosynthesis of non-linear, branched-chain acetate esters

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Cong Trinh

PROVIDER: MSV000088838 | MassIVE | Tue Feb 15 13:00:00 GMT 2022

SECONDARY ACCESSION(S): PXD031694

REPOSITORIES: MassIVE

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Proteome reallocation enables the selective de novo biosynthesis of non-linear, branched-chain acetate esters.

Seo Hyeongmin H Giannone Richard J RJ Yang Yung-Hun YH Trinh Cong T CT

Metabolic engineering 20220511

The one-carbon recursive ketoacid elongation pathway is responsible for making various branched-chain amino acids, aldehydes, alcohols, ketoacids, and acetate esters in living cells. Controlling selective microbial biosynthesis of these target molecules at high efficiency is challenging due to enzyme promiscuity, regulation, and metabolic burden. In this study, we present a systematic modular design approach to control proteome reallocation for selective microbial biosynthesis of branched-chain ...[more]

PMID: 35561848

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