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Glycoproteomics of Triple Negative Breast Cancer Cells Reveals Plexin B3 as a Cell Surface Marker That is Required for Cell Growth and Invasion

ABSTRACT: Here, we performed N-glycoproteomics on six triple negative breast cancer cell lines (commercially available cell lines: HCC1187, HCC1937, MDA-MB157, MDA-MB231, MDA-MB436, MDA-MB468) and five normal control cell lines (commercially available MCF10A and 4 non-immortalized human mammary epithelial cells: HMEC_RM10, HMEC_RM1, HMEC_RM2, HMEC_HB5) using hydrazide-based enrichment. Quantitative proteomics and integrative data mining led to the discovery of Plexin B3 (PLXNB3) as a previously undescribed TNBC-enriched cell surface protein.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Thomas Kislinger

PROVIDER: MSV000088911 | MassIVE | Thu Feb 24 05:45:00 GMT 2022

REPOSITORIES: MassIVE

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