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Proteomic and Phosphoproteomic Landscapes of Acute Myeloid Leukemia

ABSTRACT: Mass spectrometry results from TurboID experiments using constructs expressing TurboID only, NPM1wt-Turbo ID (N- and C-terminal fusions) and NPMc-Turbo ID fusions (N- and C-terminal fusions) transduced in primary mouse hematopoietic stem/progenitor cells from lineage-depleted mouse bone marrow.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Timothy J. Ley

PROVIDER: MSV000089028 | MassIVE | Wed Mar 09 17:56:00 GMT 2022

SECONDARY ACCESSION(S): PXD032176

REPOSITORIES: MassIVE

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Publications

Proteomic and phosphoproteomic landscapes of acute myeloid leukemia.

Kramer Michael H MH Zhang Qiang Q Sprung Robert R Day Ryan B RB Erdmann-Gilmore Petra P Li Yang Y Xu Ziheng Z Helton Nichole M NM George Daniel R DR Mi Yiling Y Westervelt Peter P Payton Jacqueline E JE Ramakrishnan Sai M SM Miller Christopher A CA Link Daniel C DC DiPersio John F JF Walter Matthew J MJ Townsend R Reid RR Ley Timothy J TJ

Blood 20220901 13

We have developed a deep-scale proteome and phosphoproteome database from 44 representative acute myeloid leukemia (AML) patients from the LAML TCGA dataset and 6 healthy bone marrow-derived controls. After confirming data quality, we orthogonally validated several previously undescribed features of AML revealed by the proteomic data. We identified examples of posttranscriptionally regulated proteins both globally (ie, in all AML samples) and also in patients with recurrent AML driver mutations. ...[more]

PMID: 35895896

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