Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Shigella Flexneri 5a Str. M90t (ncbitaxon:1086030)
SUBMITTER: Francois-Xavier Campbell-Valois
PROVIDER: MSV000095473 | MassIVE | Mon Jul 29 08:15:00 BST 2024
SECONDARY ACCESSION(S): PXD054349
REPOSITORIES: MassIVE
Haidar-Ahmad Nathaline N Tomaro Kyle K Lavallée-Adam Mathieu M Campbell-Valois François-Xavier F-X
mSphere 20241031 11
Promiscuous biotin ligases derived from the bacterial enzyme BirA are used to identify proteins vicinal to a bait protein, thereby defining its proxisome. Despite the popularity of this approach, surprisingly little is known about its use in prokaryotes. Here, we compared the activity of four widely used promiscuous biotin ligases in the cytoplasm of <i>Shigella flexneri</i>, a pathogenic subgroup of <i>Escherichia coli</i>. Our data indicate that the kinetics of TurboID's biotinylating activity ...[more]