Project description:The study aimed to investigate the effect of pH on Bacillus subtilis secretome into cell-free supernatant which is used as plant growth biostimulant
Project description:Frequently observed in tropical and sub-tropical regions, crops contamination by aflatoxin B1 (AFB1) produced by Aspergillus flavus, is emerging in Europe, due to climate change. Many alternative methods are currently developed to reduce the use of chemical inputs to prevent mycotoxin contamination, such as biocontrol agents (BCAs). Actinobacteria are known to produce many bioactive compounds and some of them are able to reduce in vitro AFB1 concentration. In this context, the present study aims to analyze the effect of a cell free supernatant (CFS) from Streptomyces roseolus liquid culture on A. flavus development, as well as on its transcriptome profile using microarray assay and its impact on AFB1 concentration. To study the impact of Streptomyces roseolus cell free supernatant on global transcriptome of Aspergillus flavus we have employed whole genome microarray expression profiling.
Project description:Analysis of gene expression in RAW264.7 cells stimulated for osteoclastogenesis and then treated with cell culture supernatant from Lactobacillus reuteri. Results will offer insight into targeted mechanisms suppressing osteoclastogenesis
Project description:Cell-free supernatants (CFS) of Lactobacillus johnsonii KD1 exhibited inhibitory activity against the growth of foodborne pathogenic bacteria, including the Gram-negative bacteria Vibrio parahaemolyticus 3HP and Salmonella enterica serovar Typhimurium LT2, as well as the Gram-positive bacterium Listeria monocytogenes 10403S. Therefore, the CFS of this probiotic strain was subjected to proteomic profiling to investigate potential proteins associated with antibacterial activity against foodborne pathogens.