Project description:Urine TMT-multiplexing proteomic study using high pH RP 1st dimension fractionation and 2nd dimension 1D-low pH RP LCMS analysis. Two 2D-LCMS runs in this study. Each run has 20 fractions. Urine samples are paired with MSV 000084084.
Project description:Tissue samples were collected from 61 stage I/II PDAC cases then were placed into 10% neutral buffered formalin solution, fixed at roomtemperature (8–72 h) and processed routinely into a paraffin embedded tissue block (FFPE). The RNAstorm™ Kit (biotium, USA) was used to extracted and isolated total RNA fraction from FFPE Tissue Samples. RNA libraries were prepared for sequencing using prepared SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian Kit. RNA-seq was performed by the Illumina sequencing platform on a 150-bp paired-end run.