Proteomics

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TopFD - A Software Tool for Top-Down Mass Spectrometry Feature Detection


ABSTRACT: The two data sets were generated from SW480 and SW620 colorectal cancer (CRC) cells. Proteoforms extracted from the sample were analyzed using a Thermo Q-Exactive HF mass spectrometer coupled with a 105-minute nanoRPLC separation system. The top 5 precursor ions in each MS1 spectrum were selected from an isolation window of 4 m/z for MS/MS analysis using higher-energy collisional dissociation (HCD). Both MS1 and MS/MS spectra were acquired at a resolution of 120,000 (at 200 m/z). Three technical replicates were obtained.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Liangliang Sun   Xiaowen Liu  

PROVIDER: MSV000090488 | MassIVE | Fri Oct 07 06:38:00 BST 2022

REPOSITORIES: MassIVE

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