THE EFFECT OF AGE ON THE PROTEOMIC PROFILE OF EQUINE BRONCHOALVEOLAR LAVAGE FLUID

ABSTRACT: Neonates have different cellular composition in their bronchoalveolar lavage fluid (BALF) when compared to older foals and adult horses; however, little is known about the non-cellular components of BALF. The objective of this study was to determine the proteomic composition of BALF in neonatal horses and to compare it to that of older foals and adult horses. Bronchoalveolar lavage fluid samples of seven neonates (< 1 week age), four 5 to 7-week-old foals, and six adult horses were collected. Quantitative Proteomics of the fluid was performed using tandem mass tag labeling followed by LC-MS/MS and protein relative expressions were compared between groups. A total of 704 proteins were identified with gene ontology terms and were classified. Of these, 332 proteins were related to the immune system in neonates, foals, and adult horses. The most frequent molecular functions identified were binding and catalytic activity and the most common biological processes were cellular process, metabolic process, and biological regulation. There was a significant difference in the proteome of neonates when compared to foals and to adult horses. Neonates had less relative expression (FDR < 0.01) of many immune-related proteins, including immunoglobulins, proteins involved in the complement cascade, ferritin, BPI fold-containing family B member 1, and macrophage receptor MARCO. This is the first report of neonatal foals BALF proteomics and reveals differential expression of proteins when compared to BALF from adult horses. The lower relative expression of immune-related proteins in neonates could contribute to their susceptibility to pulmonary infections

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Equus Caballus (ncbitaxon:9796)

SUBMITTER: Macarena G. Sanz  

PROVIDER: MSV000090531 | MassIVE | Thu Oct 13 13:57:00 BST 2022

SECONDARY ACCESSION(S): PXD037366

REPOSITORIES: MassIVE