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An integrated workflow for phosphopeptide identification in natural killer cells (NK) and their targets during immunological synapse formation

ABSTRACT: This protocol describes the identification and quantification of phosphopeptides during the dynamic formation of an immunological synapse. Isotope-labeled immune and target cells are mixed, cell-to-cell conjugates are stabilized by cross-linking, and isolated by fluorescence-activated cell sorting (FACS). Phosphopeptides are isolated by phosphopeptide enrichment and measured by mass spectrometry. The resulting data are analyzed to separate cell-specific phosphopeptides using the isotope label and quantified by label-free quantification (LFQ).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Daniel Perez Hernandez Gunnar Dittmar

PROVIDER: MSV000090645 | MassIVE | Fri Nov 04 06:21:00 GMT 2022

REPOSITORIES: MassIVE

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