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Dietary Grape Against Atopic Dermatitis in NC/NgaTndCrlj Mice: Proteomics Analysis

ABSTRACT: The study demonstrates the effects of dietary grape powder against atopic dermatitis in 2,4-dinitrofluorobenzene-induced atopic dermatitis in NC/NgaTndCrlj mice. To uncover molecular mechanism(s) of biological responses of grape powder, dorsal skin samples from normal control (noAD), atopic dermatitis control (ctlAD) and 5% grape powder (5GP) prevention groups were analyzed using gel-free quantitative global proteomics analysis at the School of Pharmacy Analytical Instrumentation Facility, University of Wisconsin–Madison. Briefly, sample proteins (20 micrograms) were digested with 1 microgram sequencing grade trypsin and analyzed by nano-LC/MS/MS. The data were searched against the Swiss-Prot mouse proteome database using the Sequest HT search engine in the Proteome Discoverer 1.4 software, and data were aligned using the ChromAlign algorithm. Quantitation of peptides was performed on processed data using SIEVE 2.1 (ThermoFisher Scientific).

INSTRUMENT(S): nano-LC/MS/MS

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Chandra K. Singh Nihal Ahmad

PROVIDER: MSV000090686 | MassIVE | Wed Nov 09 21:14:00 GMT 2022

REPOSITORIES: MassIVE

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