Dataset Information

Mass spectrometry based identification of similarities and differences in protein content of cutaneous melanoma cell lines and exosomes

ABSTRACT: Intercellular transport of proteins mediated by extracellular vesicles (EVs) exosomes and ectosomes, is one of the factors facilitating carcinogenesis. Therefore, the research on protein cargo of melanoma-derived EVs may provide a better understanding of mechanisms related to melanoma progression and contribute to development of novel biomarkers. The data concerning the proteome of melanoma-derived EVs is very limited. We used the shotgun nanoLC-MS/MS approach to the profile protein content of primary (WM115, WM793) and metastatic (WM2664, WM1205Lu) cutaneous melanoma cell lines and exosomes. All cell lines secreted homogeneous populations of exosomes enriched in the common set of proteins. A total of 3514 and 1234 unique proteins were identified in melanoma cells and exosomes, respectively. Gene Ontology analysis showed that many of them were involved in cancer cell proliferation, migration, escape from apoptosis, epithelial mesenchymal transition and angiogenesis. The obtained results expand the knowledge about the role of selected proteins in the biology of exosomes, as well as their functional role in the development and progression of cutaneous melanoma. The results might also be used as a starting point for further studies exploring diagnostic and prognostic potential of exosomes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Malgorzata Przybylo

PROVIDER: MSV000090907 | MassIVE | Thu Dec 15 07:01:00 GMT 2022

SECONDARY ACCESSION(S): PXD038861

REPOSITORIES: MassIVE

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Similarities and Differences in the Protein Composition of Cutaneous Melanoma Cells and Their Exosomes Identified by Mass Spectrometry.

Surman Magdalena M Jankowska Urszula U Wilczak Magdalena M Przybyło Małgorzata M

Cancers 20230208 4

Intercellular transport of proteins mediated by extracellular vesicles (EVs)-exosomes and ectosomes-is one of the factors facilitating carcinogenesis. Therefore, the research on protein cargo of melanoma-derived EVs may provide a better understanding of the mechanisms involved in melanoma progression and contribute to the development of alternative biomarkers. Proteomic data on melanoma-derived EVs are very limited. The shotgun nanoLC-MS/MS approach was applied to analyze the protein composition ...[more]

PMID: 36831440

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