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Membrane proteome analysis of FIP200-deficient human neurons

ABSTRACT: FIP200 (also known as RB1CC1) has been implicated in a number of psychiatric disorders. The current project aims to characterize the membranomic changes in neurons due to FIP200 loss of function. To this end, two isogenic human pluripotent stem cell (hPSC) lines with FIP200 KO mutations in exon 4 were generated using CRISPR-Cas9-mediated genome editing. The resulting KO clones, together with control lines, were then forward programmed into neurons by overexpression of NGN2, followed by co-culture on mouse astrocytes for 3 weeks before harvesting the cells for MS analysis.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Oliver Bruestle Michael Peitz

PROVIDER: MSV000091643 | MassIVE | Wed Apr 05 00:22:00 BST 2023

REPOSITORIES: MassIVE

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