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Air liquid interface lung tissue asthma model

ABSTRACT: nano-DESI metabolomics followed by bottom up proteomics was performed.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: ingela lanekoff

PROVIDER: MSV000091751 | MassIVE | Thu Apr 20 01:15:00 BST 2023

REPOSITORIES: MassIVE

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Publications

Interleukin-13 Treatment of Living Lung Tissue Model Alters the Metabolome and Proteome-A Nano-DESI MS Metabolomics and Shotgun Proteomics Study.

Tóth Gábor G Golubova Anastasia A Falk Alexander A Lind Sara Bergström SB Nicholas Mark M Lanekoff Ingela I

International journal of molecular sciences 20240505 9

Asthma is a chronic respiratory disease with one of the largest numbers of cases in the world; thus, constant investigation and technical development are needed to unravel the underlying biochemical mechanisms. In this study, we aimed to develop a nano-DESI MS method for the in vivo characterization of the cellular metabolome. Using air-liquid interface (ALI) cell layers, we studied the role of Interleukin-13 (IL-13) on differentiated lung epithelial cells acting as a lung tissue model. We demon ...[more]

PMID: 38732251

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