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Complexome Profiling of the chloroplast proteome from Arabidopsis thaliana

ABSTRACT: Chloroplasts are the metabolically most active compartment of mature leaf cells. Their proteins are involved in essential cellular processes, such as photosynthesis, nitrogen fixation, and fatty acid synthesis. For this, chloroplast proteins have to associate with other fellow organellar proteins to form functional units, react to changing environmental conditions, or optimize efficiency of biochemical reactions. We here investigated chloroplast protein-protein interactions by a combination of complexome profiling and cross-linking mass spectrometry (CX-MS). Different detergents and MS settings were tested for developing a new workflow, which was found to produce data of improved quality when comparted to standard complexome profiling approaches. This procedure was applied to chloroplasts acclimated to increasing light intensities to investigate the role of protein-protein interactions in the adaption to these conditions.

INSTRUMENT(S): timsTOF Pro, Q Exactive

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Hans-Peter Braun

PROVIDER: MSV000091808 | MassIVE | Thu Apr 27 06:08:00 BST 2023

SECONDARY ACCESSION(S): PXD041848

REPOSITORIES: MassIVE

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