Unknown,Transcriptomics,Genomics,Proteomics

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Identification of target genes of translation-dependent signalling in Arabidopsis


ABSTRACT: Changes ins organellar gene expression trigger retrograde signalling. Prolyl-tRNA synthetase (PRORS1) is located in chloroplasts and mitochondria. Thus, prors1-2 mutants are impaired in chloroplast and mitochondrial gene expression. The effects of the prors1-2 mutation on the global transcriptome were investigated with microarray analyses. RNA was extracted from Col-0 (WT, 4-weeks-old) and prors1-2 (38-d-old) leaves of the same developmental stage

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Tatjana Kleine 

PROVIDER: E-GEOD-54573 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of target genes and transcription factors implicated in translation-dependent retrograde signaling in Arabidopsis.

Leister Dario D   Romani Isidora I   Mittermayr Lukas L   Paieri Francesca F   Fenino Elena E   Kleine Tatjana T  

Molecular plant 20140529 7


Changes in organellar gene expression (OGE) trigger retrograde signaling. The molecular dissection of OGE-dependent retrograde signaling based on analyses of mutants with altered OGE is complicated by compensatory responses that mask the primary signaling defect and by secondary effects that influence other retrograde signaling pathways. Therefore, to identify the earliest effects of altered OGE on nuclear transcript accumulation, we have induced OGE defects in adult plants by ethanol-dependent  ...[more]

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