Proteomics

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SoDA-PAL Protein Profiling Proteomics of Sorgoleone Catabolizing Microbial Isolate Acinetobacter pittii SO1

ABSTRACT: Photoaffinity probe-based protein labeling of microbial isolate Acinetobacter pittii SO1 (NCBITAXON:48296), isolated from the sorghum rhizosphere that can utilize sorgoleone as a sole carbon source in order to identify proteins that bind sorgoleone. Photoaffinity labeling of A. pittii lysates using the SoDA-PAL probe approach may be extended in the future to proteomic profiling of complex rhizosphere microbiomes to discover genes that can be leveraged to promote beneficial plant-microbe interactions (grown on both acetate and purified sorgoleone). Eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization for subsequent quantitative proteomic analysis.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Acinetobacter Pittii (ncbitaxon:48296)

SUBMITTER: Robert G. Egbert  

PROVIDER: MSV000091986 | MassIVE | Thu May 18 10:23:00 BST 2023

SECONDARY ACCESSION(S): PXD042334

REPOSITORIES: MassIVE

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Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders in <i>Acinetobacter pittii</i>.

Van Fossen Elise M EM   Kroll Jared O JO   Anderson Lindsey N LN   McNaughton Andrew D AD   Herrera Daisy D   Oda Yasuhiro Y   Wilson Andrew J AJ   Nelson William C WC   Kumar Neeraj N   Frank Andrew R AR   Elmore Joshua R JR   Handakumbura Pubudu P   Lin Vivian S VS   Egbert Robert G RG  

Applied and environmental microbiology 20240909 10

Interactions between plants and soil microbial communities that benefit plant growth and enhance nutrient acquisition are driven by the selective release of metabolites from plant roots, or root exudation. To investigate these plant-microbe interactions, we developed a photoaffinity probe based on sorgoleone (<u>so</u>rgoleone <u>d</u>iazirine <u>a</u>lkyne for <u>p</u>hoto<u>a</u>ffinity <u>l</u>abeling, SoDA-PAL), a hydrophobic secondary metabolite and allelochemical produced in <i>Sorghum bic  ...[more]

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