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Prostate Cancer Reshapes the Secreted and Extracellular Vesicle Urinary Proteomes

ABSTRACT: Urine is a remarkably complex fluid, providing a non-invasive snapshot of an individual's overall physiologic state and of the genitourinary tissues through which it passes. Urine includes both secreted proteins and proteins encapsulated in tissue-derived extracellular vesicles (EVs). To better understand the population variability and clinical utility of urine, we comprehensively profiled both the secreted and EV proteomes of 190 men, including prostate cancer patients. We demonstrate a simple protocol to enrich for prostatic proteins in urine, and show that secreted and EV proteins reflect different subcellular compartments. Urinary proteomes are accurate surrogates of tissue proteomes, but proteins secreted from cell lines are not. The urinary proteome is longitudinally stable, and accurately distinguishes malignant from benign prostatic lesions, and can risk-stratify prostate tumors. Taken together, this resource quantifies the complexity of the urinary proteome, and reveals the synergistic value of distinguishing secreted and EV proteomes for biomarker studies.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Thomas Kislinger

PROVIDER: MSV000092061 | MassIVE | Wed May 31 07:42:00 BST 2023

REPOSITORIES: MassIVE

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