Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Monkeypox and vaccinia virus infection of primary human astrocytes

ABSTRACT: Primary human astrocytes were infected with either monkeypox virus (MPXV clade IIb lineage), vaccinia virus (VACV: Acambis 2000), or controls (MC=monkeypox control, AC = Vaccinia control) at an MOI of 10 for 6 h. Samples (n=4) were analyzed by LC-MS/MS with label-free quantification where the data was acquired by data-dependent acquisition (DDA).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Olivier Julien

PROVIDER: MSV000092229 | MassIVE | Wed Jun 21 11:58:00 BST 2023

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Monkeypox virus infection of human astrocytes causes gasdermin B cleavage and pyroptosis.

Miranzadeh Mahabadi Hajar H Lin Y C James YCJ Ogando Natacha S NS Moussa Eman W EW Mohammadzadeh Nazanin N Julien Olivier O Alto Neal M NM Noyce Ryan S RS Evans David H DH Power Christopher C

Proceedings of the National Academy of Sciences of the United States of America 20240212 8

Monkeypox virus (MPXV) infections in humans cause neurological disorders while studies of MPXV-infected animals indicate that the virus penetrates the brain. Pyroptosis is an inflammatory type of regulated cell death, resulting from plasma membrane rupture (PMR) due to oligomerization of cleaved gasdermins to cause membrane pore formation. Herein, we investigated the human neural cell tropism of MPXV compared to another orthopoxvirus, vaccinia virus (VACV), as well as its effects on immune respo ...[more]

PMID: 38346199

Similar Datasets

Insights into responses of human astrocytes to H5N1 infection by transcriptional analysis

Project description:Increasing evidence demonstrates influenza virus can not only affect the respiratory system, but also infect CNS and lead to CNS disorder and encephalopathy and encephalitis. Astrocytes are the most abundant cells in the CNS, which are capable of producing cytokines and neurotrophic factors and are essential for brain homeostasis and neuronal function. Previous studies suggested that influenza virus can infect astrocytes and induce proinflammatory cytokines response as well as apoptosis. Nevertheless, very few mechanistic data are available regarding host responses to H5N1 infection in astrocytes. In this study, a functional genomics approach was utilized to investigate comprehensive host responses to H5N1 infection in a human astrocyte cell line, U251 cells. U251 cells were infected by H5N1 at MOI 1 or control . At time points 6, 12, and 24h, total RNA were extracted for microarray experiment. Three replicates were performed at each time point of infection and control infection.

2016-01-04 | E-GEOD-66597 | biostudies-arrayexpress

Effect of small molecules on activated astrocytes

Project description:We have developed an assay to test the neuroprotective properties of compounds using stem cellM-bM-^@M-^Sderived motor neurons and astrocytes, together with activated microglia as a stress paradigm. Hit compounds were discovered and the transcriptional response on activated astrocytes cells was tested. Neural stem cell (NSC)-derived astrocytes (AC) were activated with LPS and IFN-M-NM-3 and treated with hit compound for 4 hr.

2012-11-03 | E-GEOD-37611 | biostudies-arrayexpress

Insights into responses of human astrocytes to H5N1 infection by transcriptional analysis

2016-01-04 | GSE66597 | GEO

RT-PCR analysis of HSV-1 infected mouse astrocytes and microglia

Project description:Primary mouse microglia and astrocytes were isolated from 6-8 week old C57BL/6J mice and infected with HSV-1 KOS at an MOI of 5 for 12 hours.

2020-07-11 | GSE154176 | GEO

DamID LaminB1 data in mouse MEFs, wild-type and POU2F1-/-. DamID LaminA data in mouse Neural Precursor Cells and Astrocytes.

Project description:DamID LaminB1 data were generated in POU2F1-/- MEFs to study the potential role of POU2F1/Oct1 in genome - nuclear lamina interactions. DamID LaminA data were generated in NPCs and Astrocytes to study similarities/differences between LaminA and LaminB1 binding. Comparison of MEF wt versus MEF POU2F1-/-. Comparison of LaminA (NPC & AC) with LaminB1 (NPC & AC data in GSE17051)

2012-10-10 | E-GEOD-36132 | biostudies-arrayexpress

DIRECT CONVERSION OF FIBROBLASTS INTO FUNCTIONAL ASTROCYTES BY DEFINED TRANSCRIPTION FACTORS

Project description:Direct cell reprogramming has enabled the direct conversion of skin fibroblasts into functional neurons and oligodendrocytes using a minimal set of cell lineage-specific transcription factors. This approach has substantial advantages since it is rapid and simple, generating the cell type of interest in a single step. However, it remains unknown whether this technology can be applied for directly reprogramming skin cells into astrocytes, the third neural lineage. Astrocytes play crucial roles in neuronal homeostasis and their dysfunctions contribute to the origin and progression of multiple human diseases. Herein, we carried out a screening using several transcription factors involved in defining the astroglial cell fate and identified NFIA, NFIB and SOX9 to be sufficient to convert with high efficiency embryonic and post-natal mouse fibroblasts into astrocytes (iAstrocytes). We proved both by gene expression profiling and functional tests that iAstrocytes are comparable to native brain astrocytes. This protocol can be then employed to generate functional iAstrocytes for a wide range of experimental applications. Induced astrocytes (iAstro) were compared to Fibroblasts (Fibro) as negative control and to primary astrocytes (astro) as positive control. Three biological replicates were analyzed for each experimental group.

2015-06-22 | E-GEOD-59018 | biostudies-arrayexpress

Astrocytes and microglia establish physical interactions during CNS inflammation

Project description:To investigate astrocyte-microglia interactions in vivo, we took advantage of the recombinant rabies virus (RV). We used glycoprotein G-deficient RV expressing the fluorescent protein mCherry and pseudotyped with glycoprotein EnvA, in combination with GFAP-Cre RABVgp4/TVA transgenic mice that express TVA protein and glycoprotein G in astrocytes under the control of the Gfap promoter. Following injection into GfapRABVgp4/TVA mice, RV initially infects TVA expressing astrocytes, and following its replication RV infect cells in direct contact with astrocytes. We then sorted mCherry+ or mCherry- microglia on EAE mice and prove that microglia that had interacted with astrocytes had a more proinflammatory transcriptional profile.

2022-02-25 | GSE149107 | GEO

Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles

Project description:JC polyomavirus (JCPyV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a devastating demyelinating disease of the central nervous system that results in the widespread formation of lesions across the brain parenchyma. The virus is opportunistic and remains in a benign state in the kidneys and lymphoid organs of more than half of the global human adult population. However, in rare cases of severe or selective immune suppression, JCPyV can establish a lytic infection of glial cells in the brain. While PML has traditionally been characterized as a lytic infection of oligodendrocytes, more recent findings suggest an important role for astrocytes during the initial stages of disease. Because of the exceptional species and tissue specificity of the virus, appropriate models of JCPyV infection in the brain are lacking, thus hampering progress towards the development of novel antiviral strategies and biomarkers of disease activity. Towards the aim of biomarker development extracellular vesicles (EVs) were isolated from JCPyV-infected and mock-infected human iPSC-derived astrocytes and analyzed by LC-MS/MS. As a inflammatory control, EVs were also isolated from cytokine-stimulated iPSC-derived astrocytes. We demonstrate that the proteomic signature associated with EVs from JCPyV-infected astrocytes reflect what was observed on a cellular level for infected astrocytes while being strikingly different from that of EVs generated under inflammatory conditions.

2023-08-28 | PXD044784 | Pride

Transcriptional response to flavivirus infection in neurons, astrocytes and microglia in vivo and in vitro

Project description:Bulk RNA-seq comparison of neurons, astrocytes and microglia, after infection with LGTV, TBEV and control.

2023-10-27 | E-MTAB-12902 | biostudies-arrayexpress

Mouse primary astrocytes: LCN2 protein treated vs. control

Project description:Transcriptional profiling of mouse primary astrocytes comparing control untreated astrocytes with astrocytes treated with recombinant LCN2 protein (10 micro gram/ml). Goal was to determine the effects of LCN2 treatment on global gene expression in astrocytes. A secreted protein lipocalin-2 (LCN2) has been implicated in diverse cellular processes including cell morphology and migration. We have previously demonstrated that lcn2 mediates reactive astrocytosis. In order to further understand the role of lcn2 in the CNS, astrocyte transcriptome was analyzed following LCN2 treatment. Chemokines were the major group of genes upregulated by LCN2. Two-condition experiment, control untreated astrocytes vs. LCN2 protein treated astrocytes. Biological replicates: 1 control replicates, 1 treated replicates.

2012-06-29 | E-GEOD-15667 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data