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Syphilis and the Host; multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis.

ABSTRACT: Whole cell lysates from hCMEC/d3 brain microvascular endothelial cells grown in medium and heavy SILAC media and exposed to Treponema pallidum or control media for 5 hours. Prior to Mass-spec analysis, each sample was fractionated into 12 fractions via high-pH reversed-phase liquid chromatography, then each fraction was analyzed by liquid chromatography tandem mass spectrometry. Exposures were repeated with 4 biological replicates, which we define as individual sample wells. In samples 1&2 medium SILAC cells were exposed to Treponema pallidum, and heavy SILAC cells were exposed to control media. In Samples 3&4 medium SILAC cells were exposed to control media, and heavy SILAC cells were exposed to Treponema pallidum. Cells were grown in D4-lysine (37.5 mg/liter) and 13C6-arginine (21.75 mg/liter) for medium isotope-labeled cells, or 13C615N2-lysine (38.5 mg/liter) and 13C615N4-arginine (22.25 mg/liter) for heavy isotope-labeled cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Caroline Cameron

PROVIDER: MSV000092275 | MassIVE | Mon Jun 26 16:37:00 BST 2023

SECONDARY ACCESSION(S): PXD043317

REPOSITORIES: MassIVE

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Syphilis and the Host; multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis.

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