Project description:With this experiment we sought to characterise the effect of Extracellular Vesicle (EV) mediated transfer of mitochondria from Neural Stem Cells (NSCs) to macrophages. Briefly, macrophages were obtained from the bone marrow of C57BL/6 mice and stimulated with 50 ng/ml LPS. Macrophages were treated with EVs derived from Neural Stem Cells for 6 hours after which RNA was extracted for microarray analysis.
Project description:We performed single nucleus RNA-seq on rat fetal lungs from: control (normal), hypoplastic (injury), and hypoplastic+ amniotic fluid stem cell extracellular vesicles treated (injury+treatment).
Project description:Fetal hypoplastic lungs have multilineage inflammation that is reversed by amniotic fluid stem cell extracellular vesicle treatment