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The Synthetic Coolant (WS-23) in Disposable Electronic Cigarettes Impairs Cytoskeletal Function in EpiAirwayTM Tissues Exposed at the Air Liquid Interface

ABSTRACT: WS-23 is a synthetic coolant added to disposable 4th generation electronic cigarettes at high doses (up to 40 mg/mL). Here, we show the effects of pure WS-23 on the human bronchial epithelium treated at the air-liquid-interface in a cloud chamber exposure system. Both IPA and DAVID analysis show of the affects of WS-23 are related to impairment of actin cytoskeleton signaling, cell adhesion, cell spreading, and cell movement. Follow up assays on bronchial epithetlial cell line BEAS-2B further support the impairment of actin cytoskeleton signaling and actin depolymerization, as well as inhibition of cell movement, spreading, and gap closure. These results suggest that at the concentrations of WS-23 found in popular disposable electronic cigarettes, there is likely to be an adverse effect on actin cytoskeleton signaling in bronchial epithelial cells of users.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Prue Talbot

PROVIDER: MSV000092524 | MassIVE | Tue Jul 25 16:20:00 BST 2023

REPOSITORIES: MassIVE

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