Dataset Information

Comparative analysis of differential cellular genome and proteome regulation by HIV-1 and HIV-2 pseudovirions in the early phase of infection

ABSTRACT: Beside the similar structure and genomic organization of human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2), striking difference exist between the in terms of replication dynamics and clinical manifestation of infection. Although pathomechanism of HIV-1 infection well characterized, relatively few data are available regarding HIV-2 viral replication, and its interaction with host cell protein during the early phase of infection. We utilized proteo-transcriptomic analyses to determine differential genome expression and proteomic changes induced by transduction with HIV-1 / 2 pseudovirions during eight, 12, and 26 hour time-points in HEK-293T cells. We show that alteration in the cellular milieu was indeed different between the two pseudovirions. The significantly higher number of genes altered by HIV-2 in the first two time-points, suggest a more diverse, yet subtle effect on the host cell, preparing the infected cell for integration and latency. On the other hand, GO analysis showed that, while HIV-1 induced cellular oxidative stress and had a greater effect on the cellular metabolism, HIV-2 mostly affected genes involved in cell adhesion, extracellular matrix organization or keratinocyte differentiation. Proteomics analysis revealed that HIV-1 upregulated, while HIV-2 downregulated proteins involved viral protein expression and replication. Our study provides an insight into the understudied replication cycle of HIV-2, and enrich our knowledge about the use of HIV-based lentiviral vectors in general.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Human Immunodeficiency Virus 2 (ncbitaxon:11709) Human Immunodeficiency Virus 1 (ncbitaxon:11676)

SUBMITTER: Gergo Kallo

PROVIDER: MSV000092581 | MassIVE | Thu Aug 03 04:12:00 BST 2023

SECONDARY ACCESSION(S): PXD044317

REPOSITORIES: MassIVE

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Comparative Analysis of Differential Cellular Transcriptome and Proteome Regulation by HIV-1 and HIV-2 Pseudovirions in the Early Phase of Infection.

Linkner Tamás Richárd TR Ambrus Viktor V Kunkli Balázs B Szojka Zsófia Ilona ZI Kalló Gergő G Csősz Éva É Kumar Ajneesh A Emri Miklós M Tőzsér József J Mahdi Mohamed M

International journal of molecular sciences 20231227 1

In spite of the similar structural and genomic organization of human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2), striking differences exist between them in terms of replication dynamics and clinical manifestation of infection. Although the pathomechanism of HIV-1 infection is well characterized, relatively few data are available regarding HIV-2 viral replication and its interaction with host-cell proteins during the early phase of infection. We utilized proteo-transcriptomic analyse ...[more]

PMID: 38203551

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