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Genomics and metabolomics-based assessment of the biosynthetic potential of the sponge-associated microorganism Streptomyces cacaoi strain R2A-843A from the Philippines

ABSTRACT: The biosynthetic machinery of the sponge-associated Streptomyces cacaoi strain R2A-843A was assessed using a combined genomics and metabolomics approach. Whole genome sequencing and molecular networking showed the high biosynthetic potential of this actinomycete. A significant proportion of the genome is dedicated to secondary metabolite production, with biosynthetic gene clusters for nonribosomal peptides, polyketides and terpenes being the most represented. Seven cyclic pentapeptides, including a putative new analogue, and a glycosylated lanthipeptide were identified using HRMS and untargeted MSMS analysis. Chemistry-guided purification confirmed the production of the peptides BE-18257A (1) and BE-18257B (2). The production of 1 and 2 and the growth of the microorganism were monitored for eight days. Compound 2 was produced at higher concentration, starting at 48 h post-incubation.

INSTRUMENT(S): Xevo G2 XS Tof

ORGANISM(S): Streptomyces Cacaoi (ncbitaxon:1898)

SUBMITTER: Lilibeth A. Salvador-Reyes

PROVIDER: MSV000092786 | MassIVE | Tue Aug 29 23:44:00 BST 2023

REPOSITORIES: MassIVE

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