Proteomics

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Systematic profiling of Hefeweizen ale yeast across fermentation and repitching reveals global trends in protein dynamics during brewing


ABSTRACT: Studying the genetic and molecular characteristics of brewing yeast strains is crucial for understanding their domestication history and adaptations accumulated over time in fermentation environments, and for guiding optimizations to the brewing process itself. Saccharomyces cerevisiae (brewing yeast) is amongst the most profiled organisms on the planet, yet the temporal molecular changes that underlie industrial fermentation and beer brewing remain understudied. Here, we characterized the genomic makeup of a Saccharomyces cerevisiae ale yeast widely used in the production of Hefeweizen beers, and applied shotgun mass spectrometry to systematically measure the proteomic changes throughout two fermentation cycles which were separated by 14 rounds of serial repitching.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Sp. (ncbitaxon:4935)

SUBMITTER: Maitreya J. Dunham   Edward Marcotte  

PROVIDER: MSV000092793 | MassIVE | Wed Aug 30 11:38:00 BST 2023

REPOSITORIES: MassIVE

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