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Neuronal_Notch_Exosome_YZWang_2023_Cell_Reports

ABSTRACT: Summary Extracellular vesicles (EVs) facilitate intercellular communication by transferring cargo between cells in a variety of tissues. However, how EVs achieve cell type-specific intercellular communication is still largely unknown. We found that Notch1 and Notch2 proteins are expressed on the surface of neuronal EVs that have been generated in response to neuronal excitatory synaptic activity. Notch ligands bind these EVs on the neuronal plasma membrane, trigger their internalization, activate the Notch signaling pathway, and drive the expression of Notch target genes. The generation of these neuronal EVs requires the ESCRT-associated protein Alix. Adult Alix conditional knockout mice have reduced hippocampal Notch signaling activation and glutamatergic synaptic protein expression. Thus, EVs facilitate neuron to neuron communication via the Notch receptor-ligand system in the brain.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090) Rattus Rattus (ncbitaxon:10117)

SUBMITTER: Jeffrey N. Savas, PhD

PROVIDER: MSV000093592 | MassIVE | Wed Dec 06 21:53:00 GMT 2023

SECONDARY ACCESSION(S): PXD047597

REPOSITORIES: MassIVE

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