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2023 Jardine - Populus Trichocarpa 13CO2 labeling experiment - C1 metabolism

ABSTRACT: A 13CO2 labelling experiment was performed on Populus trichocarpa using a branch enclosure for studying diurnal emissions of both 12C-methanol and 13C-methanol. Branches were supplied with 900 ppm of 13CO2. Controls in a 12C02 environment were included. After 2.5 days, treated and control leaves were removed from the plant, flash frozen under liquid nitrogen, and stored at -80C. Frozen tissues were homogenized by bead milling and extracted in methanol prior to LC-MS/MS.

INSTRUMENT(S): Agilent 1290 Series HPLC, Q Exactive

ORGANISM(S): Populus Trichocarpa (ncbitaxon:3694)

SUBMITTER: Trent Northen Kolby Jardine

PROVIDER: MSV000093760 | MassIVE | Wed Jan 03 14:51:00 GMT 2024

REPOSITORIES: MassIVE

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