Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

WVSU_MitragynaSpeciosaProjectBBRL

ABSTRACT: Mitragyna speciosa leaves were extracted with Methanol and subjected to further analysis.

INSTRUMENT(S): Xevo G2-XS QTof

ORGANISM(S): Mitragyna Speciosa (ncbitaxon:170351)

SUBMITTER: Maria Karmella Apaya

PROVIDER: MSV000093974 | MassIVE | Wed Jan 31 21:52:00 GMT 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

KratomMitragynaSpeciosa_WVSUBBRL

Project description:Mitragyna speciosa leaves were extracted with Methanol and subjected to further analysis.

2024-02-04 | MSV000094002 | MassIVE

GNPS - KratomMitragynaSpeciosa_WVSUBBRL

Project description:Mitragyna speciosa leaves were extracted with Methanol and subjected to further analysis.

2024-02-04 | MSV000094002 | GNPS

GNPS - WVSU_MitragynaSpeciosaProjectBBRL

Project description:Mitragyna speciosa leaves were extracted with Methanol and subjected to further analysis.

2024-01-31 | MSV000093974 | GNPS

WVSU_MitragynaSpeciosaProjectBBRL020824

Project description:Mitragyna Speciosa leaves were soaked in Methanol and subjected for further analysis.

2024-02-07 | MSV000094037 | MassIVE

Mitragyna Speciosa_WVSU 031524 Run

Project description:Methanol Extracts of Mitragyna Speciosa leaves (Mature) for further analysis.

2024-03-15 | MSV000094324 | MassIVE

GNPS - Mitragyna Speciosa_WVSU 031524 Run

Project description:Methanol Extracts of Mitragyna Speciosa leaves (Mature) for further analysis.

2024-03-15 | MSV000094324 | GNPS

Evaluation of Two Simultaneous Metabolomic and Proteomic Extraction Protocols Assessed by Ultra-High-Performance Liq-uid Chromatography Tandem Mass Spectrometry

Project description:Untargeted multi-omics analysis of plasma is an emerging tool for the identification of novel biomarkers for evaluating disease prognosis and for a better understanding of molecular mechanisms underlying human disease. The successful application of metabolomic and pro-teomic approaches relies on reproducibly quantifying a wide range of metabolites and proteins. Herein, we report the results of untargeted metabolomic and proteomic analyses from blood plasma samples following analyte extraction by two frequently used solvent systems: chloro-form/methanol and methanol-only. Whole blood samples were collected from participants (n=6) at University Hospital Sharjah (UHS) hospital, then plasma was separated and extracted by two methods i. methanol precipitation and, ii. 4:3 methanol:chloroform extraction. The coverage and reproducibility of the two methods were assessed by ultra-high-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS). The study revealed that metabolite extraction by methanol-only showed greater reproducibility for both metabolomic and proteomic quantifications than did methanol/chloroform, while yielding similar peptide coverage. However, coverage of extracted metabolites was higher with the methanol/chloroform precipitation.

2023-01-17 | PXD038750 | Pride

ABA is required for plant acclimation to a combination of salt and heat stress

Project description:We examined changes in steady-state transcript level in leaves of Arabidopsis plants subjected to salinity, heat stress and their combination by a transcriptome analysis of leaves.

2016-10-24 | GSE72806 | GEO

Methanol is an endogenous elicitor molecule upon senescence of detached rice leaves

Project description:During senescence of detached rice leaves, tryptophan (Trp) and Trp-derived secondary metabolites such as serotonin and 4-coumaroylserotonin accumulated in concert with methanol (MeOH) production. This senescence-induced MeOH induction was closely associated with levels of pectin methylesterase (PME)1 mRNA and PME enzyme activity. Exogenous challenge of detached rice leaves with 1% MeOH accelerated Trp and serotonin biosynthesis with induction of the corresponding genes. No other solvents including ethanol resulted in a Trp-inducing effect. This MeOH-induced Trp synthesis was positively regulated by abscisic acid but negatively regulated by cytokinin, suggesting hormonal involvement on the action of MeOH. Endogenous overproduction or suppression of MeOH either by PME1 overexpression or RNAi gene silencing revealed that PME1 overexpressing lines produced twofold higher Trp levels with elevated Trp biosynthetic gene expression, whereas RNAi lines showed twofold reduction in Trp level in healthy control rice leaves, suggesting that MeOH acts as an endogenous elicitor to enhance Trp biosynthesis. Among many transcription factors induced following MeOH treatment, the WRKY family showed significant induction patterns of which WRKY14 appeared to play a key regulatory role in MeOH-induced Trp and Trp-derived secondary metabolite biosynthesis. Total RNAs were extracted from the detached rice leaves with 1% MeOH or distilled water for 1 d, and gene expression was compared between the two groups with two replicates. DW, detached leaves in distilled water for 1 day; MeOH (2-replications), methanol treated detached leaves at the same time point as control. 2 sets of separately normalized data; DW-MeOH(1) and MeOH(2).

2011-01-03 | E-GEOD-26387 | biostudies-arrayexpress