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The dynamics and functional impact of transfer RNA repertoires during the maternal to zygotic transition in zebrafish

ABSTRACT: Embryogenesis entails dramatic shifts in mRNA translation and turnover to account for gene expression differences during proliferation and cellular differentiation. Codon identity modulates mRNA stability during early vertebrate embryogenesis, but how the composition of tRNA pools adapts to the embryo s translational demand is unknown. By quantitatively profiling the tRNA repertoires of zebrafish embryos during the maternal-to-zygotic transition, here we find that maternal and zygotic tRNA pools are distinct. We show that translational activation during embryogenesis and tRNA gene derepression are temporally coordinated by TORC1 activity, which increases at gastrulation and inactivates the RNA polymerase III repressor Maf1a/b in vivo. Reshaping of tRNA pools results in differential tRNA anticodon supply, but these changes do not alter decoding rates in zebrafish embryos. Instead, our data indicate that tRNA repertoires reflect the inherent codon bias of the zebrafish mRNA transcriptome, and tRNA levels are boosted at gastrulation to ensure efficient translation as embryos enter differentiation.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Danio Rerio (ncbitaxon:7955)

SUBMITTER: Madalena M. Reimao-Pinto

PROVIDER: MSV000094166 | MassIVE | Mon Feb 26 02:17:00 GMT 2024

REPOSITORIES: MassIVE

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