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Brain-wide alterations revealed by spatial transcriptomics and proteomics in COVID-19 infection

ABSTRACT: Understanding the pathological basis of the neurological symptoms observed following SARS-CoV2 infection is essential to optimizing outcomes and developing therapeutics. We performed proteomics profiling across eight cortical and subcortical brain regions, frontal lobe, temporal lobe, occipital lobe, hippocampus, thalamus, basal ganglia, midbrain, and pons, using postmortem brain samples from severe acute COVID-19 patients and matched controls (n=16), all preserved as formalin-fixed paraffin-embedded (FFPE) tissue. Integrating proteomics and additional transcriptomic analyses, we identified board dysregulation of mitochondrial and synaptic pathways in deep-layer excitatory neurons and changes exhibited similarities with those seen in various age-related neurodegenerative diseases, such as Parkinson's disease (PD) and Alzheimer's disease (AD).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Daniel H. Geschwind

PROVIDER: MSV000094297 | MassIVE | Tue Mar 12 14:04:00 GMT 2024

REPOSITORIES: MassIVE

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