Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of 187AA unique peptides by mass spectrometry 2

ABSTRACT: Identification of 187AA unique peptides by mass spectrometry and detection of 187AA by mass spectrometry in 4 human cells (293T, SK-hep1, ESC-derived cardiomyocytes) and 1mouse cell(R1).

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus <mouse, Genus> (ncbitaxon:10088) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: xingguo liu

PROVIDER: MSV000094368 | MassIVE | Tue Mar 19 22:00:00 GMT 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Identification of 187AA unique peptides by mass spectrometry

Project description:Identification of 187AA unique peptides by mass spectrometry and detection of 187AA by mass spectrometry in 5 human cells (293F 293T SK-hep1 ESC-derived cardiomyocytes) and 1mouse cell(R1).

2024-03-19 | MSV000094366 | MassIVE

Detection of 187AA by mass spectrometry

Project description:Identification of 187AA unique peptides by mass spectrometry and detection of 187AA by mass spectrometry in human cells (GZF2-iPSC, GZF2-hep, 293T, SK-hep1, ESC-derived cardiomyocytes, Hep-G2 and Hela).

2023-12-07 | MSV000093595 | MassIVE

Detection of 187AA by mass spectrometry

2023-12-06 | MSV000093586 | MassIVE

Detection of 187AA by mass spectrometry

Project description:Identification of 187AA unique peptides by mass spectrometry and detection of 187AA by mass spectrometry in eight human cells (GZF2-iPSC, GZF2-hep, 293T, SK-hep1, ESC-derived cardiomyocytes, Hep-G2, QSG-7701 and Hela).

2023-12-05 | MSV000093580 | MassIVE

Kinetics after JQ1 treatment in liver cancer cell

Project description:Analysis of global gene expression after BRD4 inhibition by JQ1 in liver cancer cell lines SK-Hep1 Total RNA obtained from human liver cancer cell line SK-Hep1 cells after treatment of JQ1

2016-07-20 | E-GEOD-75908 | biostudies-arrayexpress

Methyltioadenosine regulates liver cells proteome and methylproteome. Implications in liver biology and disease.

Project description:Methylthioadenosine phosphorylase (MTAP), a key enzyme in the adenine and methionine salvage pathways, catalyzes the hydrolysis of methylthioadenosine (MTA), a compound suggested to affect pivotal cellular processes in part through the regulation of protein methylation. MTAP is expressed in a wide range of cell types and tissues and its deletion is common to cancer cells and in liver injury. The aim of this study was to investigate the proteome and methyl proteome alterations triggered by MTAP deficiency in liver cells to define novel regulatory mechanisms that may explain the pathogenic processes of liver diseases. iTRAQ analysis resulted in the identification of 216 differential proteins (p<0.05) that suggest deregulation of cellular pathways as those mediated by ERK or NFκB. R-methyl proteome analysis lead to the identification of 74 differentially methylated proteins between SK-Hep1 and SK-Hep1+ cells, including 116 new methylation sites. Restoring normal MTA levels in SK-Hep1+ cells parallels the specific methylation of 56 proteins, including KRT8, TGF and CTF8A, which provides a novel regulatory mechanism of their activity with potential implications in carcinogenesis. Inhibition of RNA binding proteins methylation is especially relevant upon accumulation of MTA. As an example, methylation of quaking protein in R242 and R256 in SK-Hep1+ cells may play a pivotal role in the regulation of its activity as indicated by the up-regulation of its target protein p27 kip1. The phenotype associated with a MTAP deficiency was further verified in the liver of MTAP+/- mice. Our data support that MTAP deficiency leads to MTA accumulation and deregulation of central cellular pathways, increasing proliferation and decreasing the susceptibility to chemotherapeutic drugs, which involves differential protein methylation.

2016-02-01 | PXD002957 | Pride

Kinetics after JQ1 treatment in liver cancer cell

Project description:Analysis of global gene expression after BRD4 inhibition by JQ1 in liver cancer cell lines SK-Hep1

2016-07-20 | GSE75908 | GEO

The effect of IL-37 in HCC cell line Sk-hep1

Project description:We performed whole microarray expressiong prolife of Sk-hep1 cells which could over-express IL-37. This project is to explore the effect of interleukin-37 on hepatocellular carcinoma(HCC) development. We transfected human HCC cell line Sk-hep1 with lentiviral vector encoding IL-37 or control. The successfully transfected cells were sorted by FACS and the cells were further cultured for 24 hours. The cells were harvested and send for microarray analysis.

2020-02-06 | GSE144728 | GEO

MicroRNAs changed in response to HDGF treatment

Project description:We determined the RNAs which were suggested to commonly increase or decrease in HepG2 and SK-Hep1 cells in response to exogenously supplied hepatoma-derived growth factor (HDGF).

2022-06-01 | GSE151762 | GEO

Proteomic of M. hyorhinis-treated SK-HEP1 cells

Project description:Protein (30 μg) extracted from SK-Hep1 with or without M. hyorhinis infection

2022-10-10 | PXD037283 |