Proteomics

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Separation selectivity of small peptides on 100-120 A pore size sorbents is driven by both hydrophobic interactions and ion-pairing dependent size exclusion effects


ABSTRACT: Comparison of separation selectivity between 100A and 120A pore size C18 sorbents revealed higher peptide retentivity for the wider-pore packing material, despite it having a smaller surface area. Concurrently, peptide retention shift between 100A and 120A was more pronounced for smaller peptides. These two findings are at odds with the general notions that (1) larger surface area leads to higher retention, (2) size exclusion effects don't happen for small (<5000 Da) peptides in this pore size range and (3) size exclusion effects are directly (not inversely) proportional to analyte size. The 100A (col2) and 120A (col3) jurkat digest samples were run in triplicates.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Oleg Krokhin  

PROVIDER: MSV000094555 | MassIVE | Wed Apr 17 10:09:00 BST 2024

REPOSITORIES: MassIVE

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