Dataset Information

Comparison of affinity enrichment methods for O-GlcNAc proteomics

ABSTRACT: In this work, by using the lysates of PANC-1 (a pancreatic cancer cell line), we provided a head-to-head comparison of three affinity enrichment methods/materials (i.e., antibody, lectin AANL6, and an OGA mutant) for O-GlcNAc proteomics. The enrichment was performed before or after high-pH HPLC fractionation. The enriched peptides were analyzed by EThcD or HCD-product dependent-EThcD (i.e., HCD-pd-EThcD) mass spectrometry. The resulting data files were processed by using three different data analysis packages (i.e. Sequest HT, Byonic, and FragPipe).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Junfeng Ma

PROVIDER: MSV000094604 | MassIVE | Tue Apr 23 13:45:00 BST 2024

REPOSITORIES: MassIVE

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Systematic Evaluation of Affinity Enrichment Methods for O-GlcNAc Proteomics.

Hou Chunyan C Wu Ci C Wu Zichun Z Cheng Yifan Y Li Weiyu W Sun Hui H Ma Junfeng J

Journal of proteome research 20240920 10

O-Linked β-<i>N</i>-acetylglucosamine (O-GlcNAc) modification (i.e., O-GlcNAcylation) on proteins plays critical roles in the regulation of diverse biological processes. However, protein O-GlcNAcylation analysis, especially at a large scale, has been a challenge. So far, a number of enrichment materials and methods have been developed for site-specific O-GlcNAc proteomics in different biological settings. Despite the presence of multiple methods, their performance for the O-GlcNAc proteomics is ...[more]

PMID: 39302247

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