Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Use of MALDI-TOF mass spectrometry and IDBac to selectively mine for understudied bacterial genera from the environment.

ABSTRACT: This bacterial MALDI-TOF dataset was used for the study "Use of MALDI-TOF mass spectrometry and IDBac to selectively mine for understudied bacterial genera from the environment'. Each spectra contains up to three replicates of MALDI-MS profiles of each bacterial isolate.

INSTRUMENT(S): autoflex, rapifleX

ORGANISM(S): Not Applicable

SUBMITTER: Brian Murphy

PROVIDER: MSV000094968 | MassIVE | Mon Jun 10 10:47:00 BST 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS - Use of MALDI-TOF mass spectrometry and IDBac to selectively mine for understudied bacterial genera from the environment.

Project description:This bacterial MALDI-TOF dataset was used for the study "Use of MALDI-TOF mass spectrometry and IDBac to selectively mine for understudied bacterial genera from the environment'. Each spectra contains up to three replicates of MALDI-MS profiles of each bacterial isolate.

2024-06-10 | MSV000094968 | GNPS

Minimizing Taxonomic and Natural Product Redundancy in Microbial Libraries Using MALDI-TOF MS and the Bioinformatics Pipeline IDBac

Project description:This bacterial MALDI-TOF dataset was used for the study "AMinimizing Taxonomic and Natural Product Redundancy in Microbial Libraries Using MALDI-TOF MS and the Bioinformatics Pipeline IDBac" It consists of triplicate MALDI-TOF MS profiles (both reflectron and linear datasets) for >1,600 bacteria cultivated from a collection in Iceland.

2019-02-18 | MSV000083461 | MassIVE

Project description:Typing of Klebsiella species by FTIR and MALDI-TOF

| PRJEB27975 | ENA

Identification of Trichuris spp. using MALDI-TOF MS

Project description:Identification of different Trichuris spp. using MALDI-TOF MS

| PRJEB50468 | ENA

IDBac_Publication_MALDI-Spectra

Project description:This dataset includes MALDI spectra needed to reproduce the results and figures in "Coupling MALDI-TOF mass spectrometry protein and specialized metabolite analyses to rapidly discriminate bacterial function."

2017-10-17 | MSV000081619 | MassIVE

Addition of insoluble fiber to isolation media allows for increased diversity of lab-cultivable microbes derived from gut samples RAW DATA

Project description:This is the raw MALDI-TOF protein and small molecule data collected on 82 bacterial isolates cultivated from the intestines of zebrafish.

2019-07-05 | MSV000084061 | MassIVE

Optimization of a Top-Down Proteomics Platform for Closely-Related Pathogenic Bacteria Discrimination

Project description:The current technique used for microbial identification in hospitals is MALDI-TOF MS. However, it suffers from important limitations, in particular for closely-related species or when the database used for the identification lacks the appropriate reference. In this work, we set up a high throughput LC-MS/MS top-down proteomics platform dedicated to intact bacterial protein analysis. Using Escherichia coli as a model, all steps of the workflow were optimized: protein extraction, on-line liquid chromatographic separation, MS/MS fragmentation and data analysis. Using the optimized parameters, about 220 proteins, corresponding to more than 500 proteoforms, could be identified in a single run. We then demonstrated the suitability of the developed platform for the characterization and discrimination of enterobacterial pathogens rather undistinguishable by MALDI-TOF although leading to very different clinical outcomes. For each pathogen, we identified specific proteoforms that could potentially be used as biomarkers. We also improved the characterization of poorly described bacterial strains. Our results highlight the advantage of targeting proteoforms vs peptides for accurate bacterial characterization, and qualify top-down proteomics as a promising tool in clinical microbiology.

2020-09-24 | PXD019247 | Pride

Rapid diagnosis of SARS-CoV-2 infection by use of serum polypeptides fingerprints based on MALDI-TOF MS

Project description:To construct a rapid, high-throughput screening method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

2021-07-21 | PXD027457 |

Preliminary study of identification of Trichuris suis by MALDI-TOF MS

Project description:Preliminary study of identification of Trichuris suis by MALDI-TOF MS

| PRJEB47832 | ENA

Identification of proteins from vacuoles of red algae

Project description:Vacuoles and lysosomes are single-membrane-bound organelles involved in diverse functions such as intracellular digestion and storage or secretion of metabolites. To understand their origin, evolution and fundamental features, the identification of proteins comprising these compartments in missing links would be invaluable. So, we isolated the vacuoles from Cyanidioschyzon merolae, which is considered to be one of the most primitive photosynthetic eukaryotes, and identified 46 proteins by matrix-assisted laser desorption/ionization time of flight-mass spectrometry. Keywords: peptide mass fingerprinting, MALDI-TOF

2009-09-01 | GSE12042 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data