Proteomics

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Minimizing Taxonomic and Natural Product Redundancy in Microbial Libraries Using MALDI-TOF MS and the Bioinformatics Pipeline IDBac

ABSTRACT: This bacterial MALDI-TOF dataset was used for the study "AMinimizing Taxonomic and Natural Product Redundancy in Microbial Libraries Using MALDI-TOF MS and the Bioinformatics Pipeline IDBac" It consists of triplicate MALDI-TOF MS profiles (both reflectron and linear datasets) for >1,600 bacteria cultivated from a collection in Iceland.

INSTRUMENT(S): autoflex

ORGANISM(S): Multiple

SUBMITTER: Laura Sanchez   Brian Murphy  

PROVIDER: MSV000083461 | MassIVE | Mon Feb 18 16:57:00 GMT 2019

REPOSITORIES: MassIVE

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Minimizing Taxonomic and Natural Product Redundancy in Microbial Libraries Using MALDI-TOF MS and the Bioinformatics Pipeline IDBac.

Costa Maria S MS   Clark Chase M CM   Ómarsdóttir Sesselja S   Sanchez Laura M LM   Murphy Brian T BT  

Journal of natural products 20190723 8

Libraries of microorganisms have been a cornerstone of drug discovery efforts since the mid-1950s, but strain duplication in some libraries has resulted in unwanted natural product redundancy. In the current study, we implemented a workflow that minimizes both the natural product overlap and the total number of bacterial isolates in a library. Using a collection expedition to Iceland as an example, we purified every distinct bacterial colony off isolation plates derived from 86 environmental sam  ...[more]

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