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Plant BCL-Domain Homologues play a conserved role in SWI/SNF complex stability

ABSTRACT: These datasets present results from IP-MS experiments done in the model plant Arabidopsis. The experiments used two different bait proteins (TPF1 and BDH1) or deleted versions of BDH1 expressed under its native promoter and tagged with 3xFLAG. Immunoprecipitations of the bait proteins from inflorescence tissues was done using FLAG-bound magnetic beads, followed by FLAG peptide elution of the complex and further processing for MS analyses. Detailed information about the genetic background of each transgenic line used in each experiment can be found in the spreadsheet "BDH proteomics summary table"

INSTRUMENT(S): Orbitrap Fusion Lumos, timsTOF HT

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Javier Gallego-Bartolome

PROVIDER: MSV000094986 | MassIVE | Tue Jun 11 10:16:00 BST 2024

REPOSITORIES: MassIVE

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Mitotic bookmarking by SWI/SNF subunits.

Project description:For cells to initiate and sustain a differentiated state, it is necessary that a 'memory' of this state is transmitted through mitosis to the daughter cells1-3. Mammalian switch/sucrose non-fermentable (SWI/SNF) complexes (also known as Brg1/Brg-associated factors, or BAF) control cell identity by modulating chromatin architecture to regulate gene expression4-7, but whether they participate in cell fate memory is unclear. Here we provide evidence that subunits of SWI/SNF act as mitotic bookmarks to safeguard cell identity during cell division. The SWI/SNF core subunits SMARCE1 and SMARCB1 are displaced from enhancers but are bound to promoters during mitosis, and we show that this binding is required for appropriate reactivation of bound genes after mitotic exit. Ablation of SMARCE1 during a single mitosis in mouse embryonic stem cells is sufficient to disrupt gene expression, impair the occupancy of several established bookmarks at a subset of their targets and cause aberrant neural differentiation. Thus, SWI/SNF subunit SMARCE1 has a mitotic bookmarking role and is essential for heritable epigenetic fidelity during transcriptional reprogramming.

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ChIP-seq analysis of H-NS in Acinetobacter baumannii AB5075

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RNA immunoprecipitation to identify RHA-binding transcripts in HEK293 cells

Project description:Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from four experiments with samples obtained in four independent RNA immunoprecipitations identified 375 transcripts that co-immunoprecipitate with FLAG RHA.

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Co-immunoprecipitation of RNAs with RNA helicase A (RHA)

Project description:Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from duplicate experiments with samples obtained in two independent RNA immunoprecipitations identified 407 transcripts that co-immunoprecipitate with FLAG RHA. Keywords: gene expression array-based

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SWI/SNF Alterations in Squamous Bladder Cancers.

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SWI/SNF chromatin remodeling controls Notch-responsive enhancer accessibility.

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