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Proteomics to decipher subcellular protein features of mouse heart

ABSTRACT: Protein compartmentalisation to distinctive subcellular niches is critical for cardiac function and homeostasis. Here, we employed a rapid and robust workflow based on differential centrifugal-based fractionation with mass spectrometry (MS)-based proteomics and bioinformatic analyses for systemic mapping of the subcellular proteome of mouse heart. Using supervised machine learning (ML) of 450 hallmark protein markers from 16 subcellular niches, we further refined the subcellular information of 2083 proteins with high confidence. Our data validation focused on specific subcellular niches such as mitochondria, cell surface, cardiac dyad, and myofibril including heart tissue-enriched nuclear fraction, underscoring the significance of protein localisation in cardiac biology. This study provides novel insights into the molecular landscape of different subcellular niches of inside the heart and will serve as a draft map for heart subcellular proteome.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: David Greening

PROVIDER: MSV000095211 | MassIVE | Tue Jul 02 06:43:00 BST 2024

REPOSITORIES: MassIVE

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