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Polyomavirus ALTOs, but not MTs, downregulate viral early gene expression by activating the NF-KB pathway

ABSTRACT: Polyomaviruses are small, circular dsDNA viruses that can cause cancer. Alternative splicing of polyomavirus early transcripts generates large and small tumor antigens (LT, ST) that play essential roles in viral replication and tumorigenesis. Some polyomaviruses also express middle tumor antigens (MTs) or alternate LT open reading frames (ALTOs), which are evolutionarily related but have distinct gene structures. MTs are a splice variant of the early transcript whereas ALTOs are overprinted on the second exon of the LT transcript in an alternate reading frame and are translated via an alternative start codon. Merkel cell polyomavirus (MCPyV), the only human polyomavirus that causes cancer, encodes an ALTO but its role in the viral lifecycle and tumorigenesis has remained elusive. In this MassIVE entry, we provide mass spectrometry data and result files associated with a TurboID experiment to identify human and viral proteins that bind to ALTO.

INSTRUMENT(S): Orbitrap eclipse w/FAIMS

ORGANISM(S): Merkel Cell Polyomavirus (ncbitaxon:493803) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Denise Galloway

PROVIDER: MSV000095457 | MassIVE | Fri Jul 26 14:19:00 BST 2024

REPOSITORIES: MassIVE

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Polyomavirus ALTOs, but not MTs, downregulate viral early gene expression by activating the NF-κB pathway

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