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Alkylation reageant comparaison

ABSTRACT: Comparison of off target alkylation using chloroacetamide (CAA) and iodoacetamide (IAA). The peptide preparation was performed side by side, following identical methods except the alkylation treatment with either IAA or CAA. None of the comparison of off-targets on between IAA and CAA showed a significant difference. The overall off-target level for both reagents was relatively low (< 0.1%) and the off-target rate on lysine residues was even lower (0.045% for IAA and 0.051% for CAA).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Justin Walley

PROVIDER: MSV000095495 | MassIVE | Wed Jul 31 08:58:00 BST 2024

SECONDARY ACCESSION(S): PXD054447

REPOSITORIES: MassIVE

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Publications

Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.

Song Gaoyuan G Montes Christian C Olatunji Damilola D Malik Shikha S Ji Chonghui C Clark Natalie M NM Pu Yunting Y Kelley Dior R DR Walley Justin W JW

The Plant cell 20241121

Protein activity, abundance, and stability can be regulated by posttranslational modification including ubiquitination. Ubiquitination is conserved among eukaryotes and plays a central role in modulating cellular function, yet we lack comprehensive catalogs of proteins that are modified by ubiquitin in plants. In this study, we describe an antibody-based approach to enrich ubiquitinated peptides coupled with isobaric labeling to enable quantification of up to 18-multiplexed samples. This approac ...[more]

PMID: 39570863

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