Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Torsten Mueller
LAB HEAD: Dominic, Winter
PROVIDER: PXD005183 | Pride | 2017-05-30
REPOSITORIES: Pride
Action | DRS | |||
---|---|---|---|---|
InGel_CON_Rep1A_RAW.raw | Raw | |||
InGel_CON_Rep1A_SEARCH_Table2.msf | Msf | |||
InGel_CON_Rep1B_RAW.raw | Raw | |||
InGel_CON_Rep1B_SEARCH_Table2.msf | Msf | |||
InGel_CON_Rep1C_RAW.raw | Raw |
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Molecular & cellular proteomics : MCP 20170524 7
Reduction and alkylation of cysteine residues is part of virtually any proteomics workflow. Despite its frequent use, up to date no systematic investigation of the impact of different conditions on the outcome of proteomics studies has been performed. In this study, we compared common reduction reagents (dithiothreitol, tris-(2-carboxyethyl)-phosphine, and β-mercaptoethanol) and alkylation reagents (iodoacetamide, iodoacetic acid, acrylamide, and chloroacetamide). Using in-gel digests as well as ...[more]