Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Volatilome of Theobroma grandiflorum at Different Stages of Maturation Analyzed by HS-SPME-GC-MS

ABSTRACT: This dataset comprises the raw mass spectrometry files generated from the analysis of VOCs extracted from the pulp of Theobroma grandiflorum (Copoazu) at three distinct maturation stages (ripe, overripe, and medium). The study used HS-SPME-GC-MS. A Divinylbenzene/carbon wide-range/polydimethylsiloxane (DVB/CWR/PDMS) 80 um x 10 mm fiber was used in combination with a Gas Chromatograph HP 6890 Series equipped with an Agilent Mass Selective Detector 5973. The VOCs were separated on a capillary GC column of 5% Phenyl/95% Dimethyl Polysiloxane (30 m x 0.25 mm x 0.25 um).

INSTRUMENT(S): Gas Chromatograph HP 6890 Series equipped with an Agilent Mass Selective Detector 5973

ORGANISM(S): Theobroma Grandiflorum (ncbitaxon:108881)

SUBMITTER: Paula Galeano

PROVIDER: MSV000095666 | MassIVE | Tue Aug 20 19:20:00 BST 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

VOCS from Dendrobatids from Colombia

Project description:Samples from extraction of volatile organic compounds from frogs using two sampling methods: in vivo and sampling from the skin and a DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.

2019-08-15 | MSV000084211 | MassIVE

Theobroma grandiflorum

Project description:Pulp Influence on the Microbial Diversity During Cupuassu Fermentation

| PRJNA495382 | ENA

Brittonodoxa subpinnata polar extracts

Project description:For GC-MS analysis, plant material was extracted as following: 50 mg of frozen material was crushed and extracted with 700 uL of pre-cooled methanol (-20oC). Were added 60 uL of adonitol (0.2 mg mL-1, internal standard), with the mixture being centrifuged (10 min at 11000 g) and the supernatant transferred to glasses tubes to add chloroform (375 uL at -20oC) and ultrapure water (750 uL at 4oC), with the polar and non-polar phases being collected separately. The polar phase was derivatized using methoxyamine hydrochloride (20 mg mL-1 in pyridine; 28 uL, for two hours at 37oC) and 48 uL of MSTFA (N-Methyl-N-(trimethylsilyl) trifluoroacetamide) for 30 min at 37oC. Samples were injected (1 uL) into a gas chromatograph (6850 Network GC System - Agilent) coupled to a mass spectrometer (Agilent 5975C VL MSD) (GC-MS) equipped with the VF-5ms column (30 m, 0.25 mm, 0.25 um) and a pre-column (0.25 mm, 10 m). The initial column temperature was adjusted to 70oC for 5 min, increasing at a rate of 5oC min-1 to a final temperature of 295oC, with a total run time of 50 min, with Helium as the carrier gas.

2023-11-25 | MSV000093495 | MassIVE

Methanolic extracts from Dendrobatids from Colombia

Project description:Samples from methanolic extracts from skins of poison frogs. Split injection mode using a desorption temperature of 250C. Separation was performed initiating to 100C for 3 min, and then increasing the temperature to 190C using a rate of 10C/min and maintaining it by 1 min, afterwards incresing to 200C changing the rate to 2C/min and maintaining it by 1 min, and finally increasing to 280C, using a rate of 10C/min, and maintaing to this temperature for 3 min. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.1 mL/mi

2019-08-16 | MSV000084212 | MassIVE

GNPS_GC_MASSIVE_Malassezia_solids

Project description:Samples from extraction of volatile organic compounds from Malassezia species growing in solid mDixon media using DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.1 mL/min.

2020-04-09 | MSV000085267 | GNPS

GNPS_GC_MASSIVE_Malassezia_liquids

Project description:Samples from extraction of volatile organic compounds from Malassezia species growing in liquid mDixon media using DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.1 mL/min.

2020-08-02 | MSV000085892 | GNPS

GCMS_synthetic_dataset_fragrance_mix

Project description:Restek fragrant material standard mix. Product number 31807. dataset collected for GNPS MSHub algorithm training. Concentrations ranging from 1 mM to 0.1 uM. PDMS sampling out of liquid aqueous solution.

2018-02-23 | MSV000082093 | MassIVE

Reprogramming of human primary somatic cells by OCT4 and chemical compounds

Project description:Human induced pluripotent stem cells (hiPSCs) can be practically derived from neonatal human epidermal keratinocytes (NHEKs) transduced with OCT4, by a novel cocktail of small molecules including 5 μM PS48, 0.25 mM NaB, 0.5 μM A-83-01 and 0.5 μM PD0325901. These iPSCs are morphologically, molecularly and functionally similar to pluripotent hESCs. NHEKs were cultured in a 100 mm tissue culture dish and transduced 3 times (3-4 hours each transduction) with freshly produced lentivirus supernatants. 1,000,000 transduced NHEKs were seeded on the irradiated x-ray inactivated CF1 MEF feeder cells in a 100-mm dish and cultured in KCM and treated with 5 μM PS48, 0.25 mM NaB (Stemgent) and 0.5 μM A-83-01 (Stemgent) for 2 weeks, followed by changing half volume of media to hESCM and supplementing with 5 μM PS48, 0.25 mM NaB and 0.5 μM A-83-01 for another 2 weeks. Then cell culture media were changed to hESCM and supplemented with 5 μM PS48, 0.25 mM NaB, 0.5 μM A-83-01 and 0.5 μM PD0325901 (Stemgent) for additional four weeks.The culture was split by Accutase (Millipore) and treated with 1 μM Thiazovivin (Stemgent) in the first day after splitting. The iPSC colonies stained positive by Alexa Fluor 555 Mouse anti-Human TRA-1-81 antibody (BD Pharmingen) were picked up for expansion on feeder cells in hESCM and cultured routinely.

2010-12-03 | GSE25218 | GEO

Expression analysis in anaerobic Hanford 100H aquifer flow-through columns receiving lactate and chromate

Project description:Columns containing Hanford 100H aquifer sediment continuously infused with 5 mM lactate, 5 uM Cr(VI), and either 7.5 mM sulfate or 12 mM nitrate as an electron acceptor.

2014-04-08 | GSE56554 | GEO

Streptococcus pneumoniae

Project description:Spneu D39 wildtype grown in GM17 versus GM17 + 0.25 mM ZnSO4

| PRJNA106467 | ENA