Brittonodoxa subpinnata polar extracts
Ontology highlight
ABSTRACT:
INSTRUMENT(S): GC-MS (Agilent 6850 Network GC System /Agilent 5975C VL MSD)
ORGANISM(S): Brittonodoxa Subpinnata
SUBMITTER: 
Wilton Ricardo Sala-Carvalho
PROVIDER: MSV000093495 | MassIVE | Sat Nov 25 12:27:00 GMT 2023
REPOSITORIES: MassIVE
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Project description:For GC-MS analysis, plant material was extracted as following: 50 mg of frozen material was crushed and extracted with 700 uL of pre-cooled methanol (-20oC). Were added 60 uL of adonitol (0.2 mg mL-1, internal standard), with the mixture being centrifuged (10 min at 11000 g) and the supernatant transferred to glasses tubes to add chloroform (375 uL at -20oC) and ultrapure water (750 uL at 4oC), with the polar and non-polar phases being collected separately.
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