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MSIght: A Modular Platform for Improved Confidence in Global, Untargeted Mass Spectrometry Imaging Annotation

ABSTRACT: Mass spectrometry imaging (MSI) has gained popularity in large-scale clinical analyses due to its high sensitivity, specificity and throughput. However, global profiling experiments are often still restricted to LC-MS/MS analyses that lack spatial localization due to low throughput methods for on-tissue peptide confirmation. Parallel LC-MS/MS confirmation of extracted peptides is often utilized with MALDI-MSI; however, integration and analysis of the two data sets once again presents a bottleneck in throughput. Further, the incorporation of histological stains is critical for mapping identified peptides of interest back to relevant tissue regions, yet many software generated for this are not readily accessible to those lacking coding capabilities. Here, we present a novel platform termed MSIght, which automates the integration of these multiple modalities into an accessible and modular platform. Histological stains of tissue sections are co-registered to their respective MSI data sets to improve spatial localization and resolution of identified peptides. LC-MS/MS data of extracted peptides is used to confirm putative MSI identifications and generate confident MS images in an untargeted manner. This platform enables large-scale clinical cohorts to utilize MSI for global proteomic profiling to uncover novel biomarkers in a spatial manner, thus widely expanding the utility of MSI in clinical discovery.

INSTRUMENT(S): rapifleX, Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Lingjun Li

PROVIDER: MSV000095830 | MassIVE |

SECONDARY ACCESSION(S): PXD055780

REPOSITORIES: MassIVE

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