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MsiFlow: Automated Workflows for Reproducible and Scalable Multimodal Mass Spectrometry Imaging and Microscopy Analysis

ABSTRACT: Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry imaging (MALDI MSI) and microscopy holds potential for understanding pathological mechanisms by mapping molecular signatures from the tissue micro environment to specific cell populations. However, existing software solutions for MALDI MSI data analysis are incomplete, require programming skills and contain laborious manual steps, hindering broadly applicable, reproducible, and high throughput analysis to generate impactful biological discoveries. Here we present msiFlow, an accessible open-source, platform-independent and vendor-neutral software for end-to-end, high-throughput, transparent and reproducible analysis of multi modal imaging data. msiFlow integrates all necessary steps from raw data import to analysis visualization along with state-of-the-art and newly developed algorithms into automated workflows. Using msiFlow, we unravel the molecular heterogeneity of leukocytes in infected tissues by spatial regulation of ether-linked phospholipids containing arachidonic acid. We anticipate that msiFlow will facilitate the broad applicability of MSI in multi modal imaging to uncover context-dependent cellular regulations in disease states.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Dr. Prasad Phapale

PROVIDER: MSV000096465 | MassIVE | Tue Nov 19 02:55:00 GMT 2024

REPOSITORIES: MassIVE

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