Project description:Enzymatic Methyl-seq (EM-seq) is an enzyme-based method giving us reliable methylome data from small amounts of purified DNA. However, it is unclear whether EM-seq library can be constructed from crude cell lysate containing genomic DNA. We evaluated the quality of EM-seq libraries directly prepared from crude cell lysate.
Project description:Crude extracts of 1L cultures of fungal and bacterial strains isolated from citrus trees under high HLB pressure, grown for 1 week. All showed in vitro inhibition of Liberibacter crescens.