Project description:This dataset has been used to establish GroEL-proteotyping, a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL. This dataset contains raw data of two experiments: 1. Pure cultures of T. aromatica (sample names: TA, TB, TC) and P. putida (sample names: PA, PB, PC) were analyzed individually after in-solution digestion 2. T. aromatica and P. putida were cultivated independently and crude extracts were mixed in defined ratios. GroEL was pre-separated by SDS-PAGE (sample names: LOD_..., in-gel digestion)
Project description:Enzymatic Methyl-seq (EM-seq) is an enzyme-based method giving us reliable methylome data from small amounts of purified DNA. However, it is unclear whether EM-seq library can be constructed from crude cell lysate containing genomic DNA. We evaluated the quality of EM-seq libraries directly prepared from crude cell lysate.
Project description:Crude extracts of 1L cultures of fungal and bacterial strains isolated from citrus trees under high HLB pressure, grown for 1 week. All showed in vitro inhibition of Liberibacter crescens.