Dataset Information

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

ABSTRACT: Newcastle disease (ND) is an acute, febrile, and highly contagious disease caused by the virulent Newcastle disease virus (vNDV) worldwide. The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of vNDV. DF-1 cells and the lungs of specific pathogen free (SPF) chickens infected with the vNDV strain Herts/33 were analyzed via ultra high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 304 metabolites were significantly changed post vNDV infection, and most belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may be used for viral protein synthesis and genome amplification. Similar results were also confirmed in vivo; this is consistent with the characteristic of acute vNDV infection. The identification of these different metabolites will provide a great deal of important information to further understand the mechanism of vNDV replication and pathogenesis.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

SUBMITTER: panrao liu

PROVIDER: MTBLS1115 | MetaboLights | 2019-12-20

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MTBLS1115	Other
	FILES	Other
	a_MTBLS1115_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS1115_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt

Items per page:

1 - 5 of 8

Publications

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus.

Liu Panrao P Yin Yuncong Y Gong Yabin Y Qiu Xusheng X Sun Yingjie Y Tan Lei L Song Cuiping C Liu Weiwei W Liao Ying Y Meng Chunchun C Ding Chan C

Viruses 20191018 10

Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyz ...[more]

PMID: 31635316

Similar Datasets

Project description:Vaccination reduces morbidity and mortality from pneumonia but its effect on the tissue-level response to infection is still poorly understood. We evaluated pneumonia disease progression, acute phase response and lung gene expression profiles in mice inoculated intranasally with virulent gram-positive Streptococcus pneumoniae serotype (ST) 3, with and without prior immunization with pneumococcal polysaccharide ST 3 (PPS3), or co-immunization with PPS3 and with a low dose of lipopolysaccharide (LPS). Pneumonia severity was assessed in the acute phase, 5, 12, 24 and 48 h post-inoculation (p.i.) and the resolution phase of 7 days p.i. Primary PPS3 specific antibody production was upregulated and IgM binding to pneumococci increased in PPS3-immunized mice. Immunizations with PPS3 or PPS3 + LPS decreased bacterial recovery the lung and blood at 24 and 48 h and increased survival. Microarray analysis of whole lung RNA revealed significant changes in the acute phase protein serum amyloid A (SAA) between noninfected and infected mice, which were attenuated by immunization. SAA transcripts were higher in the liver and lungs of infected controls, and SAA protein was elevated in serum, but decreased in PPS3-immunized mice. Thus, during a virulent pneumonia infection, prior immunization with PPS3 in an IgM-dependent manner as well as co-immunization with PPS3 + LPS attenuated pneumonia severity and promoted resolution of infection, concomitant with significant regulation of cytokine gene expression in the lungs, and acute phase proteins in the lungs, liver and serum. Each lung RNA sample represented an individual mouse, creating biological repeats for each treatment. In-vivo treatments were as follows: non-infected lung (vehicle-immunized) control (n=5), infected lung (vehicle-immunized) control at 48 hr post-inoculation (n=5), PPS3 and LPS co-immunized lung at 48 hr post-inoculation (n=4) and PPS3 and LPS co-immunized lung at 7 days post-inoculation (n=4).