Dataset Information

Wax ester and lipophilic compound profiling of Euglena gracilis by gas chromatography-mass spectrometry: toward understanding of wax ester fermentation under hypoxia

ABSTRACT: Lipids are being increasingly used as biodiesel feedstock, and several saturated wax esters from Euglena gracilis are candidates for outdoor bulk production. Wax ester fermentation in Euglena is strongly increased by hypoxia, but key events underlying the metabolic shift toward wax ester biosynthesis are poorly understood. Profiling of wax esters and other lipophilic compounds is the first step for research toward the clarification of wax ester fermentation molecular mechanisms, and thus, a simple and comprehensive platform for their profiling is required. In this study, we established a profiling method for wax esters and lipophilic compounds in Euglena using gas chromatography-mass spectrometry (GC–MS). Using this method, we compared accumulation profiles of wax esters and lipophilic compounds between a wild-type Euglena Z strain and a bleached SM-ZK strain. Both the wild-type and the bleached strains contained C14:0 fatty acid-C14:0 fatty alcohol as a dominant wax ester. Wax ester fermentation initiated 4 h after the cessation of oxygen supply by halting the culture agitation resulting in linear increase and proportional changes of wax ester amounts during 24 h. However, complete anoxia by nitrogen gas aeration inhibited wax ester production and the addition of bicarbonates reversed the inhibition, suggesting that there is a need for an additional carbon source for wax ester fermentation under anoxia. Our simple method enables the investigation of metabolic responses leading to wax ester fermentation in Euglena.

INSTRUMENT(S): GCT Premier (Waters)

SUBMITTER: Takeshi Furuhashi

PROVIDER: MTBLS113 | MetaboLights | 2014-09-04

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS113	Other
	FILES	Other
	a_MTBLS113_euglena_wax_ester_research_april_2014_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS113_euglena_wax_ester_research_april_2014_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit.

Ogawa Takumi T Kashima Koji K Yuki Yoshikazu Y Mejima Mio M Kurokawa Shiho S Kuroda Masaharu M Okazawa Atsushi A Kiyono Hiroshi H Ohta Daisaku D

Scientific reports 20170712 1

Plant-based human vaccines have been actively developed in recent years, and rice (Oryza sativa L.) is one of the best candidate crops for their production and delivery. By expressing a modified cholera toxin B (CTB) subunit, we previously developed MucoRice-CTB, a rice-based vaccine against cholera, which is caused by infection of the intestine with the bacteria Vibrio cholerae. MucoRice-CTB lines have been extensively characterized by whole-genome sequencing and proteome analyses to evaluate t ...[more]

PMID: 28701756

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